ABSTRACT
Objective:To observe the effects of low intensity pulsed ultrasound(LIPUS) on the osteogenic differentiation of rat bone marrow mesenchymal stem cells(BMSCs) on titanium surface.Methods:BMSCs from Wistar rat bone marrow were respectively cultured on the flat titanium surface and the large grain blast acid etched(SLA) titanium surface,and induced by mineralization medium.Then,the cells were interfered by LIPUS and a control condition.Alkaline phosphatase(ALP) were quantitative determinated after 3 and 7 d mineralization induction respectively,ALP staining were observed after 14 d induction.Alizarin red staining were observed after 21 d mineralization induction.Osteogenic related protein and gene expressions were detected after mineralization induction.Results:ALP in culture medium of LIPUS group was higher than that of the control group after 3 d and 7 d mineralization induction(P＜0.05).LIPUS group showed stronger ALP staining and alizarin staining,and more mineralized nodules than control group.The expression of osteogenic related proteins,including Runx2,BMP2,OPN in LIPUS group increased.Osteogenic related genes expression,including ALP,Runx2,BMP2,OPN,OCN and Col-1 of the LIPUS group increased.Conclusion:The osteogenic differentiation of BMSCs on the fiat titanium surface or SLA titanium surface can be promoted by LIPUS.
ABSTRACT
OBJECTIVE@#The efficiency of antitumor immunity induced by dendritic cells (DCs) transfected with total RNA of laryngeal carcinoma cells was explored.@*METHOD@#DCs, induced from human peripheral blood mononuclear cells, were transfected with total RNA of laryngeal carcinoma cells. The specific antitumor immunity of cytotoxic T Lymphocytes (CTLs) that were actived by RNA-transfected DCs were detected by MTT methods in vitro. In vivo, antitumor-specific CTLs were subcutaneously injected into the nude mice previously. After 7 days, the laryngeal carcinoma cells were seeded and the tumor occurrence rate was observed. Tumor-loaded nude mice were treated by specific CTLs once (the treated group) or twice (the retreated group). The growth of the implanted tumor was observed too.@*RESULT@#DCs that transfected with tumor RNA can significantly active CTLs which induced antitumor-specific immune response against laryngeal carcinoma in vitro. In vivo, the tumor occurrence rate of the treatment group was predominantly reduced compared with that of the control (P < 0.01). The implanted tumor size of the treated and retreated groups were both significantly reduced (P < 0.05, P < 0.01) compared with the control too, especially the retreated ones (P < 0.05).@*CONCLUSION@#The tumor RNA loaded DCs can significantly active CTLs and the antitumor specific CTLs can both induce antitumor specific immune response against laryngeal carcinoma in vitro and inhibit the growth of the implanted tumor in vivo.
Subject(s)
Animals , Humans , Mice , Cell Line, Tumor , Cytotoxicity, Immunologic , Dendritic Cells , Allergy and Immunology , Immunotherapy, Adoptive , Methods , Laryngeal Neoplasms , Therapeutics , Mice, Nude , RNA, Neoplasm , Genetics , T-Lymphocytes, Cytotoxic , Allergy and Immunology , TransfectionABSTRACT
1:16) of the relative serum antibody has been observed in 80%, while none of this high level was observed in patients with Echovirus-25 and adenovirus dermatitis (